Review



prs314 ura3 vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc prs314 ura3 vector
    Prs314 Ura3 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314-URA3+(Plasmid+%2311004)/bio_rxiv__2025__10__30__685511-202-12-19
    Average 93 stars, based on 5 article reviews
    prs314 ura3 vector - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Construct:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..

    Subcloning:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..

    Control:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..

    Plasmid Preparation:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..

    Polymerase Chain Reaction:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..

    Amplification:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..

    Sequencing:

    Article Title: Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity
    Article Snippet: Plasmid pAK64, carrying the sis1 H34Q/K199A double mutant allele, was constructed using the QuickChange site-directed mutagenesis kit (cat. no. 200523; Stratagene, La Jolla, CA) and pGCH1 as template. .. Epitope-tagged plasmids pAK29–pAK32 were constructed by subcloning SIS1 alleles from plasmids pYW62, pYW66, pAK1, and pYW65 (see ) into the commercially available pESC-TRP vector (Stratagene, La Jolla, CA) followed by transposition of the c-myc–tagged SIS1 gene under control of its native promoter into single-copy plasmid pRS314 ( TRP1 ). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Plasmids used in this study Plasmid pAK50 was made by PCR amplification of the coding region of human type II Hsp40 gene HDJ1 ( DNAJB1 ) from the commercial vector pcDNA5/FRT/TO-DNAJB1 (Addgene, Cambridge, MA; cat. no. 032373) and subsequent insertion into pRS314 with 500 bp of 5′ and 3′ flanking sequence from SIS1 . ..



    Similar Products

    prs314  (ATCC)
    99
    ATCC prs314
    Prs314, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/Plasmid/pmc12738073-141-16-17
    Average 99 stars, based on 1 article reviews
    prs314 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs parent prs314 plasmid
    Parent Prs314 Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/BglII/pm36459441__cb2c00574_si_001-59-1-10
    Average 99 stars, based on 1 article reviews
    parent prs314 plasmid - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    93
    Addgene inc prs314 ura3 vector
    Prs314 Ura3 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314-URA3+(Plasmid+%2311004)/bio_rxiv__2025__10__30__685511-202-12-19
    Average 93 stars, based on 1 article reviews
    prs314 ura3 vector - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    86
    Kamada sh121cell harboring prs314 hator2 plasmid
    Sh121cell Harboring Prs314 Hator2 Plasmid, supplied by Kamada, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/harboring+hator2+plasmid+prs314+sh121cell/pm22496512__cb300058v_si_001-44-20-17
    Average 86 stars, based on 1 article reviews
    sh121cell harboring prs314 hator2 plasmid - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    91
    Addgene inc position 13 11
    Position 13 11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314+snu114-14+(Plasmid+%23111311)/pm40593493-581-23-58
    Average 91 stars, based on 1 article reviews
    position 13 11 - by Bioz Stars, 2026-09
    91/100 stars
      Buy from Supplier

    93
    Addgene inc alexa fluortm 568
    Alexa Fluortm 568, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314-URA3+(Plasmid+%2311004)/pm39894796-231-20-53
    Average 93 stars, based on 1 article reviews
    alexa fluortm 568 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc plasmid prs314

    Plasmid Prs314, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314-URA3+(Plasmid+%2311004)/pmc11546935-12-0-3
    Average 93 stars, based on 1 article reviews
    plasmid prs314 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc addgene plasmid

    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314-URA3+(Plasmid+%2311004)/pmc11546935-12-5-5
    Average 93 stars, based on 1 article reviews
    addgene plasmid - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc ura3 plasmid 110 p713

    Ura3 Plasmid 110 P713, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs314/pRS314-URA3+(Plasmid+%2311004)/pm38848692-241-34-57
    Average 93 stars, based on 1 article reviews
    ura3 plasmid 110 p713 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Journal: iScience

    Article Title: Grammar rules and exceptions for the language of transcriptional activation domains

    doi: 10.1016/j.isci.2024.111057

    Figure Lengend Snippet:

    Article Snippet: Plasmid pRS314 , Addgene , Addgene Plasmid # 11004.

    Techniques: Sequencing, Recombinant, Plasmid Preparation, Software